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Res Immunol. 1989 Jun-Aug;140(5-6):581-94.

A monoclonal antibody recognizes a subset of the H-2Dd mouse major class I antigens.

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Unité de Biologie Moléculaire du Gène, U277 INSERM, UAC 115 CNRS, Institut Pasteur, Paris.


Binding studies and competition experiments have shown that a monoclonal antibody (mAb) named 28-8-6 recognizes only 5 to 10% of the cell surface Dd molecules. The molecules detected by 28-8-6 mAb appear to be genuine H-2Dd antigens on the basis of their MW and isolectric points. In addition, the detectability of the subset of cell surface Dd molecules by 28-8-6 does not depend on their degree of glycosylation nor on the presence of mouse beta-2-microglobulin. Several interpretations are discussed. mAb 28-8-6 might detect a particular conformation or a particular chemical derivatization of otherwise normal H-2Dd molecules. Also, because the epitope recognized by 28-8-6 lies close to the peptide binding site, it is possible that mAb 28-8-6 recognizes a subset of Dd molecules bearing a certain category of self peptides.

[Indexed for MEDLINE]

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