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Cell Rep. 2014 May 8;7(3):623-33. doi: 10.1016/j.celrep.2014.03.063. Epub 2014 Apr 24.

Pkd1 regulates lymphatic vascular morphogenesis during development.

Author information

1
Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.
2
Department of Oncology, University Hospital of Lausanne, and Department of Biochemistry, University of Lausanne, 1066 Epalinges, Switzerland.
3
Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, 1142 Auckland, New Zealand.
4
Division of Haematology, Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000, Australia.
5
Hubrecht Institute-KNAW and UMC Utrecht, 3584CT Utrecht, the Netherlands.
6
Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: b.hogan@imb.uq.edu.au.

Abstract

Lymphatic vessels arise during development through sprouting of precursor cells from veins, which is regulated by known signaling and transcriptional mechanisms. The ongoing elaboration of vessels to form a network is less well understood. This involves cell polarization, coordinated migration, adhesion, mixing, regression, and shape rearrangements. We identified a zebrafish mutant, lymphatic and cardiac defects 1 (lyc1), with reduced lymphatic vessel development. A mutation in polycystic kidney disease 1a was responsible for the phenotype. PKD1 is the most frequently mutated gene in autosomal dominant polycystic kidney disease (ADPKD). Initial lymphatic precursor sprouting is normal in lyc1 mutants, but ongoing migration fails. Loss of Pkd1 in mice has no effect on precursor sprouting but leads to failed morphogenesis of the subcutaneous lymphatic network. Individual lymphatic endothelial cells display defective polarity, elongation, and adherens junctions. This work identifies a highly selective and unexpected role for Pkd1 in lymphatic vessel morphogenesis during development.

PMID:
24767999
PMCID:
PMC5005109
DOI:
10.1016/j.celrep.2014.03.063
[Indexed for MEDLINE]
Free PMC Article

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