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Plant Sci. 2014 Jun;223:36-44. doi: 10.1016/j.plantsci.2014.03.002. Epub 2014 Mar 11.

Changes in actin dynamics are involved in salicylic acid signaling pathway.

Author information

1
Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic; Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
2
Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic.
3
Charles University in Prague, Faculty of Science, Albertov 2038/6, 128 00 Prague 2, Czech Republic.
4
Institute of Experimental Botany, Academy of Science of the Czech Republic, Rozvojová 263, 165 02 Prague 6 - Lysolaje, Czech Republic.
5
Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
6
Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic. Electronic address: olga.valentova@vscht.cz.

Abstract

Changes in actin cytoskeleton dynamics are one of the crucial players in many physiological as well as non-physiological processes in plant cells. Positioning of actin filament arrays is necessary for successful establishment of primary lines of defense toward pathogen attack, depolymerization leads very often to the enhanced susceptibility to the invading pathogen. On the other hand it was also shown that the disruption of actin cytoskeleton leads to the induction of defense response leading to the expression of PATHOGENESIS RELATED proteins (PR). In this study we show that pharmacological actin depolymerization leads to the specific induction of genes in salicylic acid pathway but not that involved in jasmonic acid signaling. Life imaging of leafs of Arabidopsis thaliana with GFP-tagged fimbrin (GFP-fABD2) treated with 1 mM salicylic acid revealed rapid disruption of actin filaments resembling the pattern viewed after treatment with 200 nM latrunculin B. The effect of salicylic acid on actin filament fragmentation was prevented by exogenous addition of phosphatidic acid, which binds to the capping protein and thus promotes actin polymerization. The quantitative evaluation of actin filament dynamics is also presented.

KEYWORDS:

Actin dynamics; PR genes; Phosphatidic acid; Salicylic acid

PMID:
24767113
DOI:
10.1016/j.plantsci.2014.03.002
[Indexed for MEDLINE]

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