Format

Send to

Choose Destination
J Clin Microbiol. 2014 Jul;52(7):2359-64. doi: 10.1128/JCM.00594-14. Epub 2014 Apr 23.

CarbAcineto NP test for rapid detection of carbapenemase-producing Acinetobacter spp.

Author information

1
INSERM U914 Emerging Resistance to Antibiotics, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France.
2
INSERM U914 Emerging Resistance to Antibiotics, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
3
INSERM U914 Emerging Resistance to Antibiotics, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland patrice.nordmann@unifr.ch.

Abstract

Multidrug-resistant Acinetobacter baumannii isolates, particularly those that produce carbapenemases, are increasingly reported worldwide. The biochemically based Carba NP test, extensively validated for the detection of carbapenemase producers among Enterobacteriaceae and Pseudomonas spp., has been modified to detect carbapenemase production in Acinetobacter spp. A collection of 151 carbapenemase-producing and 69 non-carbapenemase-producing Acinetobacter spp. were tested using the Carba NP test and a modified Carba NP protocol (the CarbAcineto NP test) in this study. The CarbAcineto NP test requires modified lysis conditions and an increased bacterial inoculum compared to those of the original Carba NP test. The Carba NP test detects metallo-β-lactamase producers but failed to detect the production of other carbapenemase types among Acinetobacter spp. In contrast, the newly designed CarbAcineto NP test, which is rapid and reproducible, detects all types of carbapenemases with a sensitivity of 94.7% and a specificity of 100%. This cost-effective technique offers a reliable and affordable technique for identifying carbapenemase production in Acinetobacter spp., which is a marker of multidrug resistance in those species. Its use will facilitate the recognition of these carbapenemases and prevent their spread.

PMID:
24759709
PMCID:
PMC4097681
DOI:
10.1128/JCM.00594-14
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center