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Nat Neurosci. 2014 Jun;17(6):884-9. doi: 10.1038/nn.3709. Epub 2014 Apr 22.

High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor.

Author information

1
1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] Department of Pediatrics, Stanford University, Stanford, California, USA.
2
1] James H. Clark Center, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Palo Alto, California, USA.
3
1] James H. Clark Center, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Palo Alto, California, USA. [3] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.

Abstract

Accurate optical reporting of electrical activity in genetically defined neuronal populations is a long-standing goal in neuroscience. We developed Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential. ASAP1 demonstrated on and off kinetics of ∼ 2 ms, reliably detected single action potentials and subthreshold potential changes, and tracked trains of action potential waveforms up to 200 Hz in single trials. With a favorable combination of brightness, dynamic range and speed, ASAP1 enables continuous monitoring of membrane potential in neurons at kilohertz frame rates using standard epifluorescence microscopy.

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PMID:
24755780
PMCID:
PMC4494739
DOI:
10.1038/nn.3709
[Indexed for MEDLINE]
Free PMC Article
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