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Cell Calcium. 2014 Jul;56(1):1-13. doi: 10.1016/j.ceca.2014.03.004. Epub 2014 Apr 1.

The mitochondrial permeability transition pore is a dispensable element for mitochondrial calcium efflux.

Author information

1
Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Interdisciplinary Center for the Study of Inflammation (ICSI), Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara, Ferrara, Italy.
2
Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Interdisciplinary Center for the Study of Inflammation (ICSI), Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara, Ferrara, Italy. Electronic address: pnp@unife.it.

Abstract

The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of mitochondrial Ca(2+) homeostasis is not yet clear. Several studies that were based on PPIF inhibition or knock out suggested that mPTP is involved in the Ca(2+) efflux mechanism, while other observations have revealed the opposite result. The c subunit of the mitochondrial F1/FO ATP synthase has been recently found to be a fundamental component of the mPTP. In this work, we focused on the contribution of the mPTP in the Ca(2+) efflux mechanism by modulating the expression of the c subunit. We observed that forcing mPTP opening or closing did not impair mitochondrial Ca(2+) efflux. Therefore, our results strongly suggest that the mPTP does not participate in mitochondrial Ca(2+) homeostasis in a physiological context in HeLa cells.

KEYWORDS:

ATP5G1; Calcium (Ca(2+)); Cyclophilin F; Cyclosporine A (CsA); Mitochondria; Peptidyl prolyl isomerase F (PPIF); Permeability transition pore (PTP)

PMID:
24755650
PMCID:
PMC4074345
DOI:
10.1016/j.ceca.2014.03.004
[Indexed for MEDLINE]
Free PMC Article
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