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BMC Immunol. 2014 Apr 22;15:15. doi: 10.1186/1471-2172-15-15.

Streptococcus sanguinis-induced cytokine and matrix metalloproteinase-1 release from platelets.

Author information

1
Etablissement Français du Sang (EFS) Auvergne-Loire, Saint-Etienne, France. fabrice.cognasse@univ-st-etienne.fr.

Abstract

BACKGROUND:

Streptococcus sanguinis (S.sanguinis), a predominant bacterium in the human oral cavity, has been widely associated with the development of infective endocarditis. Platelets play both a haemostatic function and can influence both innate and adaptive immune responses. Previous studies have shown that S.sanguinis can interact with, and activate, platelets.

RESULTS:

The aim of this study was to determine whether S.sanguinis stimulates the release of matrix metalloproteinases (MMPs) 1, 2 and 9 and the pro-inflammatory mediators SDF-1, VEGF and sCD40L, from platelets and to subsequently pharmacologically address the release mechanism (s). S.sanguinis stimulated the release of MMP-1, SDF-1, VEGF and sCD40L from platelets and inhibitors of cyclooxygenase and phosphatidylinositol 3-kinase, and antagonists of the αIIbβ3 integrin and glycoprotein Ib, each inhibited the secretion of all factors.

CONCLUSIONS:

Therefore the release of MMP-1, SDF-1, VEGF and sCD40L occurs late in the platelet response to S.sanguinis and highlights the complex intracellular signalling pathways stimulated in response to S.sanguinis which lead to haemostasis, MMP and pro-inflammatory mediator secretion.

PMID:
24755160
PMCID:
PMC3998947
DOI:
10.1186/1471-2172-15-15
[Indexed for MEDLINE]
Free PMC Article

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