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Biochem Biophys Res Commun. 2014 May 16;447(4):683-8. doi: 10.1016/j.bbrc.2014.04.070. Epub 2014 Apr 19.

Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics.

Author information

1
Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, 2-1-14 Hoenzaka, Chuo-ku, Osaka 540-0006, Japan.
2
Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
3
Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, 2-1-14 Hoenzaka, Chuo-ku, Osaka 540-0006, Japan.
4
Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
5
Department of Pediatric Neurosurgery, Takatsuki General Hospital, Takatsuki, Japan; Division of Molecular Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
6
Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
7
Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, 2-1-14 Hoenzaka, Chuo-ku, Osaka 540-0006, Japan; Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan. Electronic address: kanemura@onh.go.jp.

Abstract

Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.

KEYWORDS:

Glioma; Induced pluripotent stem cells; Neuronal migration; PiggyBac transposon; Radial glia; Regenerative medicine

PMID:
24755070
DOI:
10.1016/j.bbrc.2014.04.070
[Indexed for MEDLINE]

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