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PLoS One. 2014 Apr 21;9(4):e93271. doi: 10.1371/journal.pone.0093271. eCollection 2014.

An analysis of the global expression of microRNAs in an experimental model of physiological left ventricular hypertrophy.

Author information

1
Experimental and Molecular Cardiovascular Laboratory and the Heart Failure and Cardiac Transplant Unit from the Cardiology Division at Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil; Post-Graduate Program in Cardiology and Cardiovascular Science, Porto Alegre, RS, Brazil; Post-Graduate Program in Genetics and Molecular Biology at the Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
2
Experimental and Molecular Cardiovascular Laboratory and the Heart Failure and Cardiac Transplant Unit from the Cardiology Division at Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil; Post-Graduate Program in Cardiology and Cardiovascular Science, Porto Alegre, RS, Brazil.
3
Experimental and Molecular Cardiovascular Laboratory and the Heart Failure and Cardiac Transplant Unit from the Cardiology Division at Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.
4
Post-Graduate Program in Cardiology and Cardiovascular Science, Porto Alegre, RS, Brazil; Post-Graduate Program in Genetics and Molecular Biology at the Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.

Abstract

BACKGROUND:

MicroRNAs (miRs) are a class of small non-coding RNAs that regulate gene expression. Studies of transgenic mouse models have indicated that deregulation of a single miR can induce pathological cardiac hypertrophy and cardiac failure. The roles of miRs in the genesis of physiological left ventricular hypertrophy (LVH), however, are not well understood.

OBJECTIVE:

To evaluate the global miR expression in an experimental model of exercise-induced LVH.

METHODS:

Male Balb/c mice were divided into sedentary (SED) and exercise (EXE) groups. Voluntary exercise was performed on an odometer-monitored metal wheels for 35 days. Various tests were performed after 7 and 35 days of training, including a transthoracic echocardiography, a maximal exercise test, a miR microarray (miRBase v.16) and qRT-PCR analysis.

RESULTS:

The ratio between the left ventricular weight and body weight was increased by 7% in the EXE group at day 7 (p<0.01) and by 11% at day 35 of training (p<0.001). After 7 days of training, the microarray identified 35 miRs that were differentially expressed between the two groups: 20 were up-regulated and 15 were down-regulated in the EXE group compared with the SED group (p = 0.01). At day 35 of training, 25 miRs were differentially expressed: 15 were up-regulated and 10 were decreased in the EXE animals compared with the SED animals (p<0.01). The qRT-PCR analysis demonstrated an increase in miR-150 levels after 35 days and a decrease in miR-26b, miR-27a and miR-143 after 7 days of voluntary exercise.

CONCLUSIONS:

We have identified new miRs that can modulate physiological cardiac hypertrophy, particularly miR-26b, -150, -27a and -143. Our data also indicate that previously established regulatory gene pathways involved in pathological LVH are not changed in physiological LVH.

PMID:
24751578
PMCID:
PMC3994002
DOI:
10.1371/journal.pone.0093271
[Indexed for MEDLINE]
Free PMC Article
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