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Carbohydr Polym. 2012 Aug 1;89(4):1117-22. doi: 10.1016/j.carbpol.2012.03.082. Epub 2012 Apr 2.

Immuno-enhancing activity of sulfated Auricularia auricula polysaccharides.

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Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
College of Plant Protection Nanjing Agricultural University, Nanjing 210095, PR China.
Department of Biomedicine Faculty of Veterinary Medicine University of Montreal 3200, Rue Sicotte, Saint Hyacinthe, Québec, Canada J2S 7C6.


The crude total Auricularia auricula polysaccharide (AAPct) was extracted by water decoction and ethanol precipitation, protein was removed to obtain total A. auricula polysaccharide (AAPt), then was graded into AAP1 and AAP2 through column chromatography. sAAPt, sAAP1 and sAAP2 were prepared by chlorosulfonic acid-pyridine method. In vitro test, the effects of sAAPt, sAAP1, sAAP2, AAPt, AAP1 and AAP2, on chicken peripheral lymphocytes proliferation were compared. The results showed that sAAPt and sAAP1 demonstrated better effect. In vivo test, 14-day-old chickens were injected respectively with sAAPt, sAAP1, AAPt and AAP1 at the first vaccination of ND vaccine, once a day for three days. On days 7, 14, 21 and 28 after the first vaccination, the peripheral lymphocytes proliferation and antibody titer were determined. The results indicated that sAAPt possessed the best efficacy and would be expected to be used as a component of a new-type immunopotentiator.

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