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Carbohydr Polym. 2012 Jul 1;89(3):942-7. doi: 10.1016/j.carbpol.2012.04.040. Epub 2012 Apr 21.

Isolation and antitumor activities of acidic polysaccharide from Gynostemma pentaphyllum Makino.

Author information

1
Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
2
Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
3
Department of Biological Science and Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
4
Department of Dermatology, Tianjin Medical University General Hospital, Tianjin 300052, China.

Abstract

Two acidic polysaccharides (GP-B1 and GP-C1) were obtained from Gynostemma pentaphyllum. The molecular weights (Mw) of the two fractions were 79 kDa for GP-B1 and 126 kDa for GP-C1. GP-B1 was composed of Gal, Ara, Man, Rha, Xyl, Glc, GalA and GlcA in a molar ration of 3.5:3.2:0.6:0.9:0.3:0.5:0.6:0.4. GP-C1 consisted of Gal, Ara, Man, Rha, Glc, and GlcA in the proportions of 2.1:1.0:0.3:0.5:0.4:0.9. Among them, GP-B1 treatment had a significant inhibitory effect on the growth of melanoma B16 in vivo and in vitro. Meanwhile GP-B1 could increase the relative spleen weight and stimulate the splenocyte proliferation alone or combined with ConA. Moreover, GP-B1 treatment induced an evident increase in the level of serum TNF-α, IFN-γ, and IL-12 and a reduction for IL-10 production. These results indicate that the antitumor effects of GP-B1 are associated with immunostimulation.

PMID:
24750884
DOI:
10.1016/j.carbpol.2012.04.040
[Indexed for MEDLINE]

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