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Carbohydr Polym. 2012 Jul 1;89(3):942-7. doi: 10.1016/j.carbpol.2012.04.040. Epub 2012 Apr 21.

Isolation and antitumor activities of acidic polysaccharide from Gynostemma pentaphyllum Makino.

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Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
Department of Biological Science and Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Department of Dermatology, Tianjin Medical University General Hospital, Tianjin 300052, China.


Two acidic polysaccharides (GP-B1 and GP-C1) were obtained from Gynostemma pentaphyllum. The molecular weights (Mw) of the two fractions were 79 kDa for GP-B1 and 126 kDa for GP-C1. GP-B1 was composed of Gal, Ara, Man, Rha, Xyl, Glc, GalA and GlcA in a molar ration of 3.5:3.2:0.6:0.9:0.3:0.5:0.6:0.4. GP-C1 consisted of Gal, Ara, Man, Rha, Glc, and GlcA in the proportions of 2.1:1.0:0.3:0.5:0.4:0.9. Among them, GP-B1 treatment had a significant inhibitory effect on the growth of melanoma B16 in vivo and in vitro. Meanwhile GP-B1 could increase the relative spleen weight and stimulate the splenocyte proliferation alone or combined with ConA. Moreover, GP-B1 treatment induced an evident increase in the level of serum TNF-α, IFN-γ, and IL-12 and a reduction for IL-10 production. These results indicate that the antitumor effects of GP-B1 are associated with immunostimulation.

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