Format

Send to

Choose Destination
Carbohydr Polym. 2012 Jun 20;89(2):694-700. doi: 10.1016/j.carbpol.2012.03.078. Epub 2012 Apr 2.

Characterization of antioxidant polysaccharides from Auricularia auricular using microwave-assisted extraction.

Author information

1
College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China; National Engineering Laboratory of Clean Technology for Leather Manufacture, Sichuan University, Chengdu 610065, PR China.
2
College of Life Sciences, Sichuan University, Chengdu 610064, PR China.
3
College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China.
4
National Engineering Laboratory of Clean Technology for Leather Manufacture, Sichuan University, Chengdu 610065, PR China.

Abstract

Influence of microwave-assisted extraction (MAE) to the characterization and corresponding antioxidant activity of polysaccharides from Auricularia auricula (AAP) was determined. According to gas chromatography-mass spectrometry, high performance size exclusion chromatography, Fourier transform infrared spectroscopy, partial acid hydrolysis, periodic acid oxidation, Smith degradation, methylation analysis and atomic force microscopy, AAP was a heteropolysaccharide (composed of glucose, galactose, mannose, arabinose and rhamnose at the molar ratio of 37.53:1:4.32:0.93:0.91) with the molecular weight of 2.77×10(4) Da, observed as a spherical lump, and the backbone of AAP was mainly composed by glucose with (1→3) linked. Moreover, AAP exhibited significant in vitro antioxidant activity in scavenging free radicals (ABTS, DPPH, superoxide and hydroxyl radicals), in lipid peroxidation and reducing power assays. The present result suggested that AAP by MAE had low molecular weight and remarkable antioxidant capability.

PMID:
24750775
DOI:
10.1016/j.carbpol.2012.03.078
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center