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J Bacteriol. 2014 Jul;196(13):2367-75. doi: 10.1128/JB.01552-14. Epub 2014 Apr 18.

Sulfolobus replication factor C stimulates the activity of DNA polymerase B1.

Author information

1
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
2
Danish Archaea Centre, Department of Biology, Copenhagen University, Copenhagen, Denmark.
3
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China huangl@sun.im.ac.cn.

Abstract

Replication factor C (RFC) is known to function in loading proliferating cell nuclear antigen (PCNA) onto primed DNA, allowing PCNA to tether DNA polymerase for highly processive DNA synthesis in eukaryotic and archaeal replication. In this report, we show that an RFC complex from the hyperthermophilic archaea of the genus Sulfolobus physically interacts with DNA polymerase B1 (PolB1) and enhances both the polymerase and 3'-5' exonuclease activities of PolB1 in an ATP-independent manner. Stimulation of the PolB1 activity by RFC is independent of the ability of RFC to bind DNA but is consistent with the ability of RFC to facilitate DNA binding by PolB1 through protein-protein interaction. These results suggest that Sulfolobus RFC may play a role in recruiting DNA polymerase for efficient primer extension, in addition to clamp loading, during DNA replication.

PMID:
24748616
PMCID:
PMC4054168
DOI:
10.1128/JB.01552-14
[Indexed for MEDLINE]
Free PMC Article

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