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J Vis Exp. 2014 Apr 5;(86). doi: 10.3791/50269.

Endotracheal intubation in mice via direct laryngoscopy using an otoscope.

Author information

1
Medical and Research Services, VA San Diego Healthcare System; Department of Medicine, University of California, San Diego.
2
Department of Medicine, University of California, San Diego.
3
School of Medicine, University of California, San Diego.
4
Medical and Research Services, VA San Diego Healthcare System; Department of Medicine, University of California, San Diego; tbigby@ucsd.edu.

Abstract

Mice, both wildtype and transgenic, are the principal mammalian model in biomedical research currently. Intubation and mechanical ventilation are necessary for whole animal experiments that require surgery under deep anesthesia or measurements of lung function. Tracheostomy has been the standard for intubating the airway in these mice to allow mechanical ventilation. Orotracheal intubation has been reported but has not been successfully used in many studies because of the substantial technical difficulty or a requirement for highly specialized and expensive equipment. Here we report a technique of direct laryngoscopy using an otoscope fitted with a 2.0 mm speculum and using a 20 G intravenous catheter as an endotracheal tube. We have used this technique extensively and reliably to intubate and conduct accurate assessments of lung function in mice. This technique has proven safe, with essentially no animal loss in experienced hands. Moreover, this technique can be used for repeated studies of mice in chronic models.

PMID:
24747695
PMCID:
PMC4162455
DOI:
10.3791/50269
[Indexed for MEDLINE]
Free PMC Article

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