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Infect Genet Evol. 2014 Jul;25:20-7. doi: 10.1016/j.meegid.2014.04.004. Epub 2014 Apr 16.

Molecular analysis of non structural rotavirus group A enterotoxin gene of bovine origin from India.

Author information

1
Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India. Electronic address: malikys@ivri.res.in.
2
Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India. Electronic address: navyog.yadav84@gmail.com.
3
Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India. Electronic address: kuldeepsharma@rediffmail.com.
4
Department of Hygiene, Sapporo Medical University School of Medicine, S 1, W 17, Chuo-Ku, Sapporo, Hokkaido 060-8556, Japan. Electronic address: souvikrota@gmail.com.
5
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary. Electronic address: bkrota@hotmail.com.
6
National Institute of Cholera and Enteric Diseases (NICED), Kolkata, West Bengal, India. Electronic address: ganeshvirologist@yahoo.co.in.
7
Sapporo Medical University School of Medicine, Chuo-Ku, Sapporo, Japan. Electronic address: koba103161@yahoo.co.jp.
8
Laboratory for Clinical and Epidemiological Virology, KU Leuven, REGA Institute, Minderbroedersstraat 10, B3000 Leuven, Belgium. Electronic address: jelle.matthijnssens@gmail.com.

Abstract

The rotavirus enterotoxin NSP4 (nonstructural protein 4), plays a pivotal role in viral morphogenesis as well as pathogenesis. In this study, the NSP4 gene of rotavirus group A (RVA) isolates of bovine origin isolated in several states of India from 2008 to 2011 were characterized. The complete open reading frame of 23 RVA strains were sequenced and analyzed phylogenetically. Genotype E1 was detected for the first time in bovines from India, in addition to the more common bovine genotype E2. Sequence similarity analysis of the E1 sequences showed a close genetic relatedness to human strains. Six of the bovine E2 genotypes strains clustered near bovine and unusual human strains (possible human animal reassortant) from Thailand, while the remaining E2 sequences clustered with Indian bovine strains. Analysis pointed out one positively selected site (154aa), believe to be part of an antigenic region and 123 negatively selected sites. Unexpectedly, a pentameric NSP4 structure of the coiled coil domain in the E1 carrying strains and a monomeric NSP4 in RVA strain P14 (E2) was predicted based on homology modeling, potentially affecting the biological properties of NSP4. The close relationship between bovine and human rotavirus strains further highlights the complex interaction among rotaviruses of different species.

KEYWORDS:

Bovine rotavirus; Evolution; Genotypes; India; Nonstructural protein 4; Selection analysis

PMID:
24747605
DOI:
10.1016/j.meegid.2014.04.004
[Indexed for MEDLINE]

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