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J Vis Exp. 2014 Apr 10;(86). doi: 10.3791/51329.

A murine model of myocardial ischemia-reperfusion injury through ligation of the left anterior descending artery.

Author information

1
Davis Heart & Lung Research Institute, Department of Physiology and Cell Biology, The Ohio State University.
2
Davis Heart & Lung Research Institute, Department of Physiology and Cell Biology, The Ohio State University; Noah.Weisleder@osumc.edu.

Abstract

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms at work during MI and developing effective therapeutic approaches requires methodology to reproducibly simulate the clinical incidence and reflect the pathophysiological changes associated with MI. Here, we describe a surgical method to induce MI in mouse models that can be used for short-term ischemia-reperfusion (I/R) injury as well as permanent ligation. The major advantage of this method is to facilitate location of the left anterior descending artery (LAD) to allow for accurate ligation of this artery to induce ischemia in the left ventricle of the mouse heart. Accurate positioning of the ligature on the LAD increases reproducibility of infarct size and thus produces more reliable results. Greater precision in placement of the ligature will improve the standard surgical approaches to simulate MI in mice, thus reducing the number of experimental animals necessary for statistically relevant studies and improving our understanding of the mechanisms producing cardiac dysfunction following MI. This mouse model of MI is also useful for the preclinical testing of treatments targeting myocardial damage following MI.

PMID:
24747599
PMCID:
PMC4080806
DOI:
10.3791/51329
[Indexed for MEDLINE]
Free PMC Article

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