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Nat Neurosci. 2014 Jun;17(6):801-3. doi: 10.1038/nn.3706. Epub 2014 Apr 20.

The age and genomic integrity of neurons after cortical stroke in humans.

Author information

1
1] Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden. [2] Department of Neurology, University of Erlangen-Nuremberg, Erlangen, Germany. [3].
2
1] Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden. [2].
3
Department of Physics and Astronomy, Ion Physics, Uppsala University, Sweden.
4
Department of Neurology, University of Erlangen-Nuremberg, Erlangen, Germany.
5
Lund Stem Cell Center, University Hospital, Lund, Sweden.
6
Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
7
Department of Neuropathology, Institute of Pathology, University of Debrecen, Medical and Health Science Centre, Debrecen, Hungary.
8
Department of Neurology, University of Debrecen, Medical and Health Science Centre, Debrecen, Hungary.
9
Department of Pathology, University Hospital, Lund, Sweden.
10
Department of Gene Technology, Royal Institute of Technology, SciLifeLab, Stockholm, Sweden.
11
1] Center for Hematology and Regenerative Medicine, Karolinska University Hospital, Huddinge, Sweden. [2] Department of Medicine, Skaraborgs Sjukhus Skövde, Sweden.
12
Deep Sequencing Group, SFB655, Biotechnology Center, TU-Dresden, Dresden, Germany.
13
Department of Mathematics, Institut Camille Jordan, Université de Lyon, Villeurbanne cedex, France.

Abstract

It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test-derived (14)C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating the relevance of an intact genome for survival.

PMID:
24747576
DOI:
10.1038/nn.3706
[Indexed for MEDLINE]

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