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DNA Repair (Amst). 2014 Jul;19:84-94. doi: 10.1016/j.dnarep.2014.03.023. Epub 2014 Apr 18.

The contribution of co-transcriptional RNA:DNA hybrid structures to DNA damage and genome instability.

Author information

1
Department of Chemical, Systems Biology, Stanford University School of Medicine, 318 Campus Drive, Stanford, CA 94305-5441, USA.
2
Department of Chemical, Systems Biology, Stanford University School of Medicine, 318 Campus Drive, Stanford, CA 94305-5441, USA. Electronic address: cimprich@stanford.edu.

Abstract

Accurate DNA replication and DNA repair are crucial for the maintenance of genome stability, and it is generally accepted that failure of these processes is a major source of DNA damage in cells. Intriguingly, recent evidence suggests that DNA damage is more likely to occur at genomic loci with high transcriptional activity. Furthermore, loss of certain RNA processing factors in eukaryotic cells is associated with increased formation of co-transcriptional RNA:DNA hybrid structures known as R-loops, resulting in double-strand breaks (DSBs) and DNA damage. However, the molecular mechanisms by which R-loop structures ultimately lead to DNA breaks and genome instability is not well understood. In this review, we summarize the current knowledge about the formation, recognition and processing of RNA:DNA hybrids, and discuss possible mechanisms by which these structures contribute to DNA damage and genome instability in the cell.

KEYWORDS:

APOBEC family; ASF splicing factor; Activation-induced deaminase (AID); Double-strand breaks; G-quadruplex; Genome instability; R-loops; RNA processing factors; RNA:DNA helicases; RNase H; Senataxin; THO/TREX complex; THSC/TREX-2; Topoisomerase; Transcription–replication conflicts; mRNP biogenesis

PMID:
24746923
PMCID:
PMC4051866
DOI:
10.1016/j.dnarep.2014.03.023
[Indexed for MEDLINE]
Free PMC Article
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