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Cell Stem Cell. 2014 Jun 5;14(6):777-80. doi: 10.1016/j.stem.2014.03.015. Epub 2014 Apr 17.

Human somatic cell nuclear transfer using adult cells.

Author information

1
Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA; CHA Stem Cell Institute, CHA University, Seoul 135-081, Korea. Electronic address: ychung@chahealthsystems.com.
2
CHA Stem Cell Institute, CHA University, Seoul 135-081, Korea.
3
Department of Biomedical Science, CHA University 135-081, Seoul 135-081, Korea.
4
CHA Fertility Center, Los Angeles, CA 90036, USA.
5
Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA.
6
Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ 07920, USA.
7
Department of Veterinary Physiology & Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
8
Advanced Cell Technology, Marlborough, MA 01752, USA.
9
Stem Cell Source LLC, Houston, TX 77056, USA.
10
Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA; CHA Stem Cell Institute, CHA University, Seoul 135-081, Korea; Department of Biomedical Science, CHA University 135-081, Seoul 135-081, Korea. Electronic address: drleedr@cha.ac.kr.

Abstract

Derivation of patient-specific human pluripotent stem cells via somatic cell nuclear transfer (SCNT) has the potential for applications in a range of therapeutic contexts. However, successful SCNT with human cells has proved challenging to achieve, and thus far has only been reported with fetal or infant somatic cells. In this study, we describe the application of a recently developed methodology for the generation of human ESCs via SCNT using dermal fibroblasts from 35- and 75-year-old males. Our study therefore demonstrates the applicability of SCNT for adult human cells and supports further investigation of SCNT as a strategy for regenerative medicine.

PMID:
24746675
DOI:
10.1016/j.stem.2014.03.015
[Indexed for MEDLINE]
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