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Methods Mol Biol. 2014;1150:247-62. doi: 10.1007/978-1-4939-0512-6_17.

Direct lineage conversion of pancreatic exocrine to endocrine Beta cells in vivo with defined factors.

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1
Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center Boston, Boston, MA, USA.

Abstract

Pancreatic exocrine cells can be directly converted to insulin(+) beta cells by adenoviral-mediated expression of three transcription factors Pdx1, Mafa, and Ngn3 in the adult mouse pancreas (Zhou et al., Nature 455(7213):627-632, 2008). This direct reprogramming approach offers a strategy to replenish beta-cell mass and may be further developed as a potential future treatment for diabetes. Here, we provide a detailed protocol for inducing exocrine to beta-cell reprogramming in mice. We also describe key analyses we routinely use to assess the phenotype and function of reprogrammed cells.

PMID:
24744004
DOI:
10.1007/978-1-4939-0512-6_17
[Indexed for MEDLINE]
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