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Eur J Nutr. 2015 Mar;54(2):183-92. doi: 10.1007/s00394-014-0699-7. Epub 2014 Apr 18.

The effect of high-fat--high-fructose diet on skeletal muscle mitochondrial energetics in adult rats.

Author information

1
Department of Biology, University of Naples, Complesso Universitario Monte Sant'Angelo, Edificio 7, Via Cinthia, 80126, Naples, Italy.

Abstract

PURPOSE:

To study the effect of isoenergetic administration to adult rats of high-fat or high-fat--high-fructose diet for 2 weeks on skeletal muscle mitochondrial energetic.

METHODS:

Body and skeletal muscle composition, energy balance, plasma lipid profile and glucose tolerance were measured, together with mitochondrial functionality, oxidative stress and antioxidant defense.

RESULTS:

Rats fed high-fat--high-fructose diet exhibited significantly higher plasma triglycerides and non-esterified fatty acids, together with significantly higher plasma glucose and insulin response to glucose load. Skeletal muscle triglycerides and ceramide were significantly higher in rats fed high-fat--high-fructose diet. Skeletal muscle mitochondrial energetic efficiency and uncoupling protein 3 content were significantly higher, while adenine nucleotide translocase content was significantly lower, in rats fed high-fat or high-fat--high-fructose diet.

CONCLUSIONS:

The results suggest that a high-fat--high-fructose diet even without hyperphagia is able to increase lipid flow to skeletal muscle and mitochondrial energetic efficiency, with two detrimental effects: (a) energy sparing that contributes to the early onset of obesity and (b) reduced oxidation of fatty acids and lipid accumulation in skeletal muscle, which could generate insulin resistance.

PMID:
24743896
DOI:
10.1007/s00394-014-0699-7
[Indexed for MEDLINE]

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