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Int J Mol Sci. 2014 Apr 16;15(4):6441-52. doi: 10.3390/ijms15046441.

The effects of the CCR6/CCL20 biological axis on the invasion and metastasis of hepatocellular carcinoma.

Author information

1
College of Life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, China. sdhzdds@163.com.
2
Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an 223300, Jiangsu, China. yeahliu007@163.com.
3
Department of General Surgery, The First Affiliated Hospital of Soochow University, No. 188, Shizi Street, Suzhou 215006, Jiangsu, China. zxdeng@163.com.
4
Department of General Surgery, Zhangjiagang Hospital Affiliated to Soochow University, Zhangjiagang 215600, Jiangsu, China. doczhangbo@163.com.
5
Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an 223300, Jiangsu, China. tangxiaojuntxj@126.com.
6
Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an 223300, Jiangsu, China. zhangti1982@126.com.
7
Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an 223300, Jiangsu, China. liuwendong@126.com.

Abstract

Chemokines and their receptors have recently been shown to play major roles in cancer metastasis. Chemokine receptor 6 (CCR6) and its ligand, CCL20, were highly expressed in a variety of human cancers. In our present study, we aimed to clarify whether CCR6/CCL20 was correlated with the migration of hepatocellular carcinoma (HCC). RT-PCR and Western blot results showed that CCR6 was overexpressed in different invasive potential HCC cell lines (p<0.05), while the expression of CCL20 had no obvious difference (p>0.05). CCR6 was suppressed by siRNA in HCCLM6, and then the biological behaviors of HCCLM6 cells were observed. The results showed that the CCR6/CCL20 biological axis increased the capacity of proliferation and adhesion, as well as the chemotactic migration and the level of cytokines related to degraded extracellular matrix. In conclusion, these findings indicate that CCR6 indeed participates in regulating the migration and invasion of HCC, and it might become a prognostic factor of HCC.

PMID:
24743888
PMCID:
PMC4013639
DOI:
10.3390/ijms15046441
[Indexed for MEDLINE]
Free PMC Article

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