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Cell Death Dis. 2014 Apr 17;5:e1178. doi: 10.1038/cddis.2014.159.

Synergistic effects of glycated chitosan with high-intensity focused ultrasound on suppression of metastases in a syngeneic breast tumor model.

Author information

1
Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
2
Department of Radiotherapy, Cheng Hsin General Hospital, Taipei, Taiwan.
3
Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
4
Division of Radiation Oncology, Taipei City Hospital RenAi Branch, Taipei, Taiwan.
5
Department of Life Sciences, TzuChi University, Hualien, Taiwan.
6
Graduate Institute of Biotechnology, Chinese Culture University, Taipei 11114, Taiwan.
7
1] Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan [2] Division of Radiation Oncology, Taipei City Hospital RenAi Branch, Taipei, Taiwan [3] Biophotonics and Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei 112, Taiwan.
8
Biophotonics Research Laboratory, Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK 73034, USA.

Abstract

Stimulation of the host immune system is crucial in cancer treatment. In particular, nonspecific immunotherapies, when combined with other traditional therapies such as radiation and chemotherapy, may induce immunity against primary and metastatic tumors. In this study, we demonstrate that a novel, non-toxic immunoadjuvant, glycated chitosan (GC), decreases the motility and invasion of mammalian breast cancer cells in vitro and in vivo. Lung metastatic ratios were reduced in 4T1 tumor-bearing mice when intratumoral GC injection was combined with local high-intensity focused ultrasound (HIFU) treatment. We postulate that this treatment modality stimulates the host immune system to combat cancer cells, as macrophage accumulation in tumor lesions was detected after GC-HIFU treatment. In addition, plasma collected from GC-HIFU-treated tumor-bearing mice exhibited tumor-specific cytotoxicity. We also investigated the effect of GC on epithelial-mesenchymal transition-related markers. Our results showed that GC decreased the expression of Twist-1 and Slug, proto-oncogenes commonly implicated in metastasis. Epithelial-cadherin, which is regulated by these genes, was also upregulated. Taken together, our current data suggest that GC alone can reduce cancer cell motility and invasion, whereas GC-HIFU treatment can induce immune responses to suppress tumor metastasis in vivo.

PMID:
24743733
PMCID:
PMC4001313
DOI:
10.1038/cddis.2014.159
[Indexed for MEDLINE]
Free PMC Article
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