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Biophys J. 2014 Apr 15;106(8):1630-7. doi: 10.1016/j.bpj.2013.11.4507.

Imaging the lipid-phase-dependent pore formation of equinatoxin II in droplet interface bilayers.

Author information

1
Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
2
Department of Chemistry, Oxford University, Oxford, UK.
3
Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia; National Institute of Chemistry, Ljubljana, Slovenia. Electronic address: gregor.anderluh@ki.si.
4
Department of Chemistry, Oxford University, Oxford, UK. Electronic address: mark.wallace@chem.ox.ac.uk.

Abstract

Using phase-separated droplet interface bilayers, we observe membrane binding and pore formation of a eukaryotic cytolysin, Equinatoxin II (EqtII). EqtII activity is known to depend on the presence of sphingomyelin in the target membrane and is enhanced by lipid phase separation. By imaging the ionic flux through individual pores in vitro, we observe that EqtII pores form predominantly within the liquid-disordered phase. We observe preferential binding of labeled EqtII at liquid-ordered/liquid-disordered domain boundaries before it accumulates in the liquid-disordered phase.

PMID:
24739162
PMCID:
PMC4008837
DOI:
10.1016/j.bpj.2013.11.4507
[Indexed for MEDLINE]
Free PMC Article

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