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Eur Ann Allergy Clin Immunol. 2014 Mar;46(2):68-73.

Molecular diagnosis and the Italian Board for ISAC.

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Section of Allergy and Clinical Immunology, Dept. of Internal Medicine, University of Bari, Bari, Italy.
Allergy Unit, Dept. of Respiratory and Allergic Diseases, Azienda Ospedaliera Umberto I, Torrette, Ancona, Italy.
Institute of Translational Pharmacology, Italian National Research Council, Second University of Naples, Naples, Italy.
Section of Immunoallergology, University of Florence, Florence, Italy.
Laboratories of Analysis, Istituto Giannina Gaslini, Genoa, Italy.
Allergy and Respiratory Diseases, IRCCS IST San Martino, University of Genoa, Genoa, Italy.
Allergy Unit, Azienda Ospedale Università, Verona, Italy.
Immunoallergology Unit, University of Salerno, Salerno, Italy.


The Component Resolved Diagnostic (CRD) approach has been developed when highly purified or recombinant allergen molecules have become available. These molecules are the allergenic proteins toward which the specific and clinically relevant IgE immune response is directed. So, the identification of protein families and cross-reactivity patterns of importance in allergy have been possible. The Italian advisory BOARD for ISAC was born: to evaluate the advantages, disadvantages and placement in diagnosis of CRD studying its application in allergic patients; to facilitate the interpretation of molecular diagnostics for clinical allergists; to evaluate the effectiveness of CRD in improving diagnostic risk assessment and early preventive treatment of allergic diseases. In the last years, its fields of interest have been: the evaluation of the performance of CRD on multi-sensitized allergic patients with respiratory symptoms and on poly-sensitized athletes; the evolution of IgE repertoire directed to single allergenic components by evaluating allergic patients with different age at a molecular level; the relevance of results obtained using allergen microarray technique for describing the IgE repertoire in allergic patients by reviewing the main articles focused on CRD published in the last 2 years; the need for an educational program focused on this new diagnostic tool also through the creation of an exhaustive and interactive explanation of the laboratory report molecular allergy; the investigation of the performance and potential additional diagnostic values of the ISAC microarray in a real-life clinical setting, taking into account also the economic values.


Component Resolved Diagnostic; Immuno Solid-phase Allergen Chip (ISAC); Italian Board for ISAC

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