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Biol Cell. 1989;65(3):305-8.

Cytokeratin analysis of breast and leukemia tumor cell lines by flow cytometry.

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1
Laboratoire de Biologie, Centre René Huguenin de Lutte contre le Cancer, Saint-Cloud, France.

Abstract

Using four human tumor cell lines, MCF-7 and T47-D from breast tumors, MOLT-4 and K-562 from leukemia, flow cytometric DNA analysis of pure and mixed cell population was performed using monoclonal antibodies to cytokeratin to distinguish cytokeratin-containing carcinoma cells from leukemia cells which do not contain cytokeratins. Surprisingly, on pure or mixed K-562 cells, we found positive labeling with KL1, CK8, and CK18 antibodies (results confirmed by immunocytology). This preliminary study has allowed a DNA analysis on epithelial cells of human breast tumors.

PMID:
2473821
[Indexed for MEDLINE]

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