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PLoS One. 2014 Apr 15;9(4):e94650. doi: 10.1371/journal.pone.0094650. eCollection 2014.

Long-read sequencing of chicken transcripts and identification of new transcript isoforms.

Author information

1
The Gladstone Institutes, San Francisco, California, United States of America; Department of Epidemiology and Biostatistics, University of California San Francisco, San Francisco, California, United States of America.
2
University of Washington, Seattle, Washington, United States of America; Pacific Biosciences, Menlo Park, California, United States of America.
3
Pacific Biosciences, Menlo Park, California, United States of America.

Abstract

The chicken has long served as an important model organism in many fields, and continues to aid our understanding of animal development. Functional genomics studies aimed at probing the mechanisms that regulate development require high-quality genomes and transcript annotations. The quality of these resources has improved dramatically over the last several years, but many isoforms and genes have yet to be identified. We hope to contribute to the process of improving these resources with the data presented here: a set of long cDNA sequencing reads, and a curated set of new genes and transcript isoforms not currently represented in the most up-to-date genome annotation currently available to the community of researchers who rely on the chicken genome.

PMID:
24736250
PMCID:
PMC3988055
DOI:
10.1371/journal.pone.0094650
[Indexed for MEDLINE]
Free PMC Article

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