Anti-miR-135b Exerts an Antitumor Effect In Vivo in the AOM/DSS Model
(A–C) Overview of the study (A): AOM was given once, followed by periodic administration of DSS in water. MiR-135b-AMO or scrambled-AMO were given twice a week for 100 days. Mice were treated with miR-135b-AMO (n = 8), scrambled-AMO (n = 8), or left untreated (n = 8). Following euthanization, macroscopic (B) and microscopic (C) analysis of the tumors was performed. Scale bars, 200 um (top), 100 um (bottom).
(D) Statistical analysis of tumor number (multiplicity, a), tumor number for tumors >2 mm (b), tumor volume (load, c), average size (d), and tumor size distribution in the two groups (e).
(E and F) Cancer tissues from mice treated with miR-135b-AMO or scrambled-AMO were analyzed. (E) Ki-67 expression was assessed with immunohistochemistry (a) and quantitated (b; scale bars, 200 um [top], 100 um [bottom]). (F) Apoptosis was assessed by immunofluorescence (scale bar, 200 um).
See also .