Format

Send to

Choose Destination
See comment in PubMed Commons below
Plant Physiol Biochem. 2014 Jul;80:90-6. doi: 10.1016/j.plaphy.2014.03.020. Epub 2014 Mar 31.

MicroRNAs involving in cold, wounding and salt stresses in Triticum aestivum L.

Author information

1
College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
2
College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China. Electronic address: kangzs@nwsuaf.edu.cn.

Abstract

MicroRNAs (miRNAs) play critical roles in post-transcriptional regulation and act as important endogenous regulators to various stresses. Cold, wounding and high-salinity are three common environmental stress stimuli influencing crops growth and development. In this study, we identified 31 known miRNAs and 3 novel miRNAs in wheat. Moreover, 19 stress-regulated miRNAs using RT-qPCR data in which the effects of three stresses were surveyed from the known miRNAs. Among them, 16, 12 and 8 miRNAs were regulated under cold, wounding and high-salinity treatments, respectively. Of which 4 miRNAs were highly responsive to cold stress in wheat by northern blot, and 6 wounding-regulated and 3 high-salinity-regulated miRNAs were detected. Meanwhile, miR159, miR393 and miR398 were responsive to multiple stress stimuli. Besides, 2 novel miRNAs were regulated by cold stress. While, the analyses of targets suggested miR159, miR398 and miR6001 could responses to stress conditions in regulation pathways. Taken together, the results of this study suggest that wheat miRNAs may play important roles in response to abiotic stress.

KEYWORDS:

MiRNAs; Northern blot; Stress; Target; Wheat

PMID:
24735552
DOI:
10.1016/j.plaphy.2014.03.020
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center