Format

Send to

Choose Destination
Front Immunol. 2014 Mar 28;5:129. doi: 10.3389/fimmu.2014.00129. eCollection 2014.

Splenic B-1a Cells Expressing CD138 Spontaneously Secrete Large Amounts of Immunoglobulin in Naïve Mice.

Author information

1
Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research , Manhasset, NY , USA.
2
Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research , Manhasset, NY , USA ; Departments of Medicine and Molecular Medicine, Hofstra North Shore-LIJ School of Medicine , Manhasset, NY , USA.

Abstract

B-1a cells constitutively secrete natural antibody that provides immediate protection against microbial pathogens and functions homeostatically to speed removal of apoptotic cell debris. Although B-1a cells are especially prominent in the peritoneal and pleural cavities, some B-1a cells reside in the spleen. A small subset of splenic B-1a cells in naïve, unimmunized mice express CD138, a recognized plasma cell antigen, whereas the bulk of splenic B-1a cells are CD138 negative. Splenic B-1a cells in toto have been shown to generate much more antibody per cell than peritoneal B-1a cells; however, specific functional information regarding CD138(+) splenic B-1a cells has been lacking. Here, we find a higher proportion of CD138(+) splenic B-1a cells spontaneously secrete more IgM as compared to CD138(-) B-1a cells. Moreover, IgM secreted by CD138(+) splenic B-1a cells is skewed with respect to N-region addition, and some aspects of VH and JH utilization, as compared to CD138(-) splenic B-1a cells and peritoneal B-1a cells. The small population of CD138(+) splenic B-1a cells is likely responsible for a substantial portion of natural IgM and differs from IgM produced by other B-1a cell subsets.

KEYWORDS:

B lymphocytes; B-1 cells; IgM; antibody

Supplemental Content

Full text links

Icon for Frontiers Media SA Icon for PubMed Central
Loading ...
Support Center