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Phytochemistry. 2014 Jul;103:114-122. doi: 10.1016/j.phytochem.2014.03.028. Epub 2014 Apr 11.

Stable, water extractable isothiocyanates from Moringa oleifera leaves attenuate inflammation in vitro.

Author information

1
Department of Plant Biology & Pathology, Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ 08901, USA. Electronic address: waterman@aesop.rutgers.edu.
2
Department of Plant Biology & Pathology, Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ 08901, USA.
3
Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC 28081, USA.

Abstract

Moringa (Moringa oleifera Lam.) is an edible plant used as both a food and medicine throughout the tropics. A moringa concentrate (MC), made by extracting fresh leaves with water, utilized naturally occurring myrosinase to convert four moringa glucosinolates into moringa isothiocyanates. Optimum conditions maximizing MC yield, 4-[(α-L-rhamnosyloxy)benzyl]isothiocyanate, and 4-[(4'-O-acetyl-α-L-rhamnosyloxy)benzyl]isothiocyanate content were established (1:5 fresh leaf weight to water ratio at room temperature). The optimized MC contained 1.66% isothiocyanates and 3.82% total polyphenols. 4-[(4'-O-acetyl-α-L-rhamnosyloxy)benzyl]isothiocyanate exhibited 80% stability at 37°C for 30 days. MC, and both of the isothiocyanates described above significantly decreased gene expression and production of inflammatory markers in RAW macrophages. Specifically, both attenuated expression of iNOS and IL-1β and production of nitric oxide and TNFα at 1 and 5 μM. These results suggest a potential for stable and concentrated moringa isothiocyanates, delivered in MC as a food-grade product, to alleviate low-grade inflammation associated with chronic diseases.

KEYWORDS:

4-[(4′-O-acetyl-α-L-rhamnosyloxy)benzyl]isothiocyanate; 4-[(α-L-rhamnosyloxy)benzyl]isothiocyanate; Chronic inflammation; Isothiocyanates; Moringa oleifera; Moringaceae

PMID:
24731259
PMCID:
PMC4071966
DOI:
10.1016/j.phytochem.2014.03.028
[Indexed for MEDLINE]
Free PMC Article
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