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PLoS One. 2014 Apr 11;9(4):e94282. doi: 10.1371/journal.pone.0094282. eCollection 2014.

B Lymphocyte Stimulator (BLyS) is expressed in human adipocytes in vivo and is related to obesity but not to insulin resistance.

Author information

1
Department I of Internal Medicine, University of Kiel, Kiel, Germany.
2
Department of General, Abdominal, Thoracic and Transplantation Surgery, University of Kiel, Kiel, Germany.
3
Department for Internal Medicine, Clinic for Endocrinology and Nephrology, University of Leipzig, Leipzig, Germany.
4
Department of General, Visceral and Cancer Surgery, University of Cologne, Cologne, Germany.
5
Institute of Medical Informatics and Statistics, University of Kiel, Kiel, Germany.

Abstract

Inflammation and metabolism have been shown to be evolutionary linked and increasing evidence exists that pro-inflammatory factors are involved in the pathogenesis of obesity and type 2 diabetes. Until now, most data suggest that within adipose tissue these factors are secreted by cells of the innate immune system, e. g. macrophages. In the present study we demonstrate that B lymphocyte stimulator (BLyS) is increased in human obesity. In contrast to several pro-inflammatory factors, we found the source of BLyS in human adipose tissue to be the adipocytes rather than immune cells. In grade 3 obese human subjects, expression of BLyS in vivo in adipose tissue is significantly increased (p<0.001). Furthermore, BLyS serum levels are elevated in grade 3 human obesity (862.5+222.0 pg/ml vs. 543.7+60.7 pg/ml in lean controls, p<0.001) and are positively correlated to the BMI (r = 0.43, p<0.0002). In the present study, bariatric surgery significantly altered serum BLyS concentrations. In contrast, weight loss due to a very-low-calorie-formula-diet (800 kcal/d) had no such effect. To examine metabolic activity of BLyS, in a translational research approach, insulin sensitivity was measured in human subjects in vivo before and after treatment with the human recombinant anti-BLyS antibody belimumab. Since BLyS is known to promote B-cell proliferation and immunoglobulin secretion, the present data suggest that adipocytes of grade 3 obese human subjects are able to activate the adaptive immune system, suggesting that in metabolic inflammation in humans both, innate and adaptive immunity, are of pathophysiological relevance.

PMID:
24728308
PMCID:
PMC3984119
DOI:
10.1371/journal.pone.0094282
[Indexed for MEDLINE]
Free PMC Article

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