Format

Send to

Choose Destination
PLoS One. 2014 Apr 11;9(4):e94369. doi: 10.1371/journal.pone.0094369. eCollection 2014.

Crystal structure of the shrimp proliferating cell nuclear antigen: structural complementarity with WSSV DNA polymerase PIP-box.

Author information

1
Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México.
2
Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México.
3
National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York, United States of America.
4
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología-Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
5
Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y Estudios Avanzados, Irapuato, Guanajuato, México.

Abstract

DNA replication requires processivity factors that allow replicative DNA polymerases to extend long stretches of DNA. Some DNA viruses encode their own replicative DNA polymerase, such as the white spot syndrome virus (WSSV) that infects decapod crustaceans but still require host replication accessory factors. We have determined by X-ray diffraction the three-dimensional structure of the Pacific white leg shrimp Litopenaeus vannamei Proliferating Cell Nuclear Antigen (LvPCNA). This protein is a member of the sliding clamp family of proteins, that binds DNA replication and DNA repair proteins through a motif called PIP-box (PCNA-Interacting Protein). The crystal structure of LvPCNA was refined to a resolution of 3 Å, and allowed us to determine the trimeric protein assembly and details of the interactions between PCNA and the DNA. To address the possible interaction between LvPCNA and the viral DNA polymerase, we docked a theoretical model of a PIP-box peptide from the WSSV DNA polymerase within LvPCNA crystal structure. The theoretical model depicts a feasible model of interaction between both proteins. The crystal structure of shrimp PCNA allows us to further understand the mechanisms of DNA replication processivity factors in non-model systems.

PMID:
24728082
PMCID:
PMC3984155
DOI:
10.1371/journal.pone.0094369
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center