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Nat Methods. 2014 Jun;11(6):625-8. doi: 10.1038/nmeth.2925. Epub 2014 Apr 13.

Rapid adaptive optical recovery of optimal resolution over large volumes.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
2
Coleman Technologies, Inc., Newtown Square, Pennsylvania, USA.
3
Division of Biology, California Institute of Technology, Pasadena, California, USA.
4
Institute of Neuronal Cell Biology, Technische Universität München, Munich, Germany.
5
1] Institute of Neuronal Cell Biology, Technische Universität München, Munich, Germany. [2] Munich Center for Systems Neurology, Munich, Germany. [3] German Center for Neurodegenerative Diseases, Munich, Germany.
6
1] Department of Cellular Biology and Anatomy, Georgia Regents University, Augusta, Georgia, USA. [2].
7
1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2].

Abstract

Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.

PMID:
24727653
PMCID:
PMC4069208
DOI:
10.1038/nmeth.2925
[Indexed for MEDLINE]
Free PMC Article

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