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Colloids Surf B Biointerfaces. 2014 Jun 1;118:83-9. doi: 10.1016/j.colsurfb.2014.03.039. Epub 2014 Mar 31.

Influence of sulfate and phosphate on the deposition of plasmid DNA on silica and alumina-coated surfaces.

Author information

1
The Key Laboratory of Water and Sediment Sciences, Ministry of Education; College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China.
2
Department of Mineral Resources and Energy Engineering, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.
3
The Key Laboratory of Water and Sediment Sciences, Ministry of Education; College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China. Electronic address: tongmeiping@pku.edu.cn.

Abstract

The influence of sulfate and phosphate on the deposition kinetics of plasmid DNA on solid surfaces was examined at a constant 300mM ionic strength in both NaCl-Na2SO4 and NaCl-NaH2PO4-Na2HPO4 mixing solutions with varied sulfate and phosphate concentrations at pH 6.0 by utilizing a quartz crystal microbalance with dissipation (QCM-D). Two representative solid surfaces, both silica and alumina-coated surfaces, were concerned in this study. To better understand the effects of sulfate and phosphate on plasmid DNA deposition, QCM-D data were complemented by diffusion coefficients and zeta potentials of DNA as a function of examined solution conditions. The presence of sulfate and phosphate in solutions decreased the deposition efficiencies of plasmid DNA on both silica and alumina-coated surfaces. Moreover, the deposition efficiencies decreased with increasing concentrations of sulfate/phosphate. With sulfate/phosphate ions present in solutions, the deposition kinetics of plasmid DNA on both silica and alumina-coated surfaces were mainly controlled by classic Derjaguin-Landau-Verwey-Overbeek (DLVO) interactions.

KEYWORDS:

Alumina; Phosphate; Plasmid DNA; QCM-D; Silica; Sulfate

PMID:
24727552
DOI:
10.1016/j.colsurfb.2014.03.039
[Indexed for MEDLINE]
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