Influence of sulfate and phosphate on the deposition of plasmid DNA on silica and alumina-coated surfaces

Colloids Surf B Biointerfaces. 2014 Jun 1:118:83-9. doi: 10.1016/j.colsurfb.2014.03.039. Epub 2014 Mar 31.

Abstract

The influence of sulfate and phosphate on the deposition kinetics of plasmid DNA on solid surfaces was examined at a constant 300mM ionic strength in both NaCl-Na2SO4 and NaCl-NaH2PO4-Na2HPO4 mixing solutions with varied sulfate and phosphate concentrations at pH 6.0 by utilizing a quartz crystal microbalance with dissipation (QCM-D). Two representative solid surfaces, both silica and alumina-coated surfaces, were concerned in this study. To better understand the effects of sulfate and phosphate on plasmid DNA deposition, QCM-D data were complemented by diffusion coefficients and zeta potentials of DNA as a function of examined solution conditions. The presence of sulfate and phosphate in solutions decreased the deposition efficiencies of plasmid DNA on both silica and alumina-coated surfaces. Moreover, the deposition efficiencies decreased with increasing concentrations of sulfate/phosphate. With sulfate/phosphate ions present in solutions, the deposition kinetics of plasmid DNA on both silica and alumina-coated surfaces were mainly controlled by classic Derjaguin-Landau-Verwey-Overbeek (DLVO) interactions.

Keywords: Alumina; Phosphate; Plasmid DNA; QCM-D; Silica; Sulfate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum Oxide / chemistry*
  • Coated Materials, Biocompatible / chemistry*
  • DNA / metabolism*
  • Diffusion
  • Osmolar Concentration
  • Phosphates / chemistry*
  • Plasmids / metabolism*
  • Polylysine / chemistry
  • Silicon Dioxide / chemistry*
  • Solutions
  • Static Electricity
  • Sulfates / chemistry*
  • Surface Properties

Substances

  • Coated Materials, Biocompatible
  • Phosphates
  • Solutions
  • Sulfates
  • Polylysine
  • Silicon Dioxide
  • DNA
  • Aluminum Oxide