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J Dent. 2014 Sep;42(9):1115-23. doi: 10.1016/j.jdent.2014.03.018. Epub 2014 Apr 12.

Effects of the application sequence of calcium-containing desensitising pastes during etch-and-rinse adhesive restoration.

Author information

1
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.
2
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China; Department of Prosthodontics, Stomatology Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, People's Republic of China.
3
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China. Electronic address: huangcui@whu.edu.cn.

Abstract

OBJECTIVES:

To evaluate the effects of different application sequence of calcium-containing desensitising pastes on bonding effectiveness and tubule occlusion during etch-and-rinse (E&R) adhesive restoration.

METHODS:

Seventy molars were sectioned parallel to the occlusal plane, polished and randomly divided into seven groups (n=10). Group 1 was etched with 35% phosphoric acid for 15s. Groups 2-4 were treated with different calcium-containing desensitisers, including an arginine-calcium carbonate (Arg-CaCO3)-containing paste, a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP)-containing paste and a calcium-sodium phosphosilicate (Novamin)-containing paste, respectively. Afterward, these groups were etched with 35% phosphoric acid for 15s. Groups 5-7 were initially etched and then treated with Arg-CaCO3-, CPP-ACP- and Novamin-containing desensitisers, respectively. In each group, samples were equally distributed into two subgroups (n=5) to bond with either a two-step E&R adhesive Adper SingleBond 2 (SB) or a three-step E&R adhesive Adper ScotchBond Multi-Purpose (SBMP). The microtensile bond strengths (MTBS) were tested and fracture modes were analyzed by stereomicroscopy and field-emission scanning electron microscopy (FESEM). Eight additional dentine disks were prepared to evaluate tubule occlusion prior to bonding using FESEM.

RESULTS:

The application sequence of calcium-containing desensitising pastes did not significantly affect MTBS irrespective of two-step SB (P>0.05) or three-step SBMP E&R adhesives (P>0.05). Effective dentinal tubule occlusion was observed in the mode of etching-desensitising.

CONCLUSIONS:

Applying calcium-containing desensitisers (particularly Arg-CaCO3- and Novamin-based) after etching during E&R adhesive restoration could achieve effective tubule occlusion without affecting the bonding strength.

KEYWORDS:

Application sequence; Dentine bonding; Dentine hypersensitivity; Desensitising paste; Etch-and-rinse adhesive

PMID:
24727119
DOI:
10.1016/j.jdent.2014.03.018
[Indexed for MEDLINE]
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