Format

Send to

Choose Destination
Fertil Steril. 2014 Jun;101(6):1637-48.e1-5. doi: 10.1016/j.fertnstert.2014.02.050. Epub 2014 Apr 14.

Atypical embryo phenotypes identified by time-lapse microscopy: high prevalence and association with embryo development.

Author information

1
Auxogyn, Menlo Park, California.
2
Pacific Fertility Center, San Francisco, California.
3
Reproductive Science Center of the Bay Area, San Ramon, California.
4
Fertility Physicians of Northern California, Palo Alto Medical Foundation, San Jose, California.
5
Stanford Fertility and Reproductive Medicine Center, Palo Alto, California.
6
Auxogyn, Menlo Park, California. Electronic address: sshen@auxogyn.com.

Abstract

OBJECTIVE:

To characterize atypical dynamic embryo phenotypes identified by time-lapse microscopy, evaluate their prevalence, and determine their association with embryo development.

DESIGN:

Retrospective multicenter cohort study.

SETTING:

Five IVF clinics in the United States.

PATIENT(S):

Sixty-seven women undergoing IVF treatment with 651 embryos.

INTERVENTION(S):

Embryo videos were retrospectively analyzed for atypical phenotypes.

MAIN OUTCOME MEASURE(S):

Identification of four groups of atypical embryo phenotypes: abnormal syngamy (AS), abnormal first cytokinesis (A1(cyt)), abnormal cleavage (AC), and chaotic cleavage (CC). Prevalence and association with embryo morphology and development potential were evaluated.

RESULT(S):

A high prevalence of atypical phenotypes was observed among embryos: AS 25.1% (163/649), A1(cyt) 31.0% (195/639), AC 18% (115/639) and CC 15% (96/639). A high percentage of embryos with atypical phenotype(s) had good quality on day 3 (overall grade good or fair): AS 78.6% (70/89); A1(cyt) 79.7% (94/119), AC 86.4% (70/81), and CC 35.2% (19/54), but the blastocyst formation rates for these embryos were significantly lower compared with their respective control groups: AS 21.5% vs. 44.9%, A1(cyt) 21.7% vs. 44.6%, AC 11.7% vs. 43.1%, and CC 14.0% vs. 42.3%.

CONCLUSION(S):

Embryos exhibiting atypical phenotypes are highly prevalent in human embryos and show significantly lower developmental potential than control embryos.

CLINICAL TRIAL REGISTRATION NUMBER:

NCT01369446.

KEYWORDS:

Atypical phenotype; abnormal embryo development; embryo selection; embryo viability assessment; time-lapse microscopy

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center