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Vet Microbiol. 2014 Jun 25;171(1-2):66-73. doi: 10.1016/j.vetmic.2014.03.009. Epub 2014 Mar 18.

Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer.

Author information

1
Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Japan.
2
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan. Electronic address: m-nagai@cc.tuat.ac.jp.
3
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
4
Department of Virology and Parasitology, School of Medicine, Fujita Health University, Toyoake, Japan.
5
Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Japan.

Abstract

There are few reports describing diarrhea of adult cattle caused by group A rotaviruses. Here, we report the identification of a novel bovine group A rotavirus from diarrhea of adult cows. A group A rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in Japan in 2013. The comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination G15P[14]. The genome constellation of this strain, namely, RVA/Cow-wt/JPN/Tottori-SG/2013/G15P[14] was G15-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3 representing VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5, respectively. Each gene segment of Tottori-SG was most closely related to Japanese bovine group A rotaviruses suggesting that Tottori-SG might have derived from multiple reassortment events from group A rotavirus strains circulating among Japanese cattle. No other diarrhea pathogen of adult cattle was detected by routine diagnosis and metagenomics. Viral metagenomics, using a next-generation sequencer, is useful to characterize group A rotaviruses from fecal samples and offers unbiased comprehensive investigations of pathogen.

KEYWORDS:

Adult cow diarrhea; Japan; Novel genotype; Rotavirus; Viral metagenomics

PMID:
24725447
DOI:
10.1016/j.vetmic.2014.03.009
[Indexed for MEDLINE]

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