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Eur J Neurol. 2014 Aug;21(8):1096-1101. doi: 10.1111/ene.12435. Epub 2014 Apr 12.

Identification of the major HHV-6 antigen recognized by cerebrospinal fluid IgG in multiple sclerosis.

Author information

1
MS Unit, Departments of Immunology and Neurology, Hospital Ramón y Cajal, IRYCIS, Madrid, Spain.
2
Red Española de Esclerosis Múltiple, Madrid, Spain.
3
Department of Neurology, Hospital Clínico San Carlos, IdISSC, Madrid, Spain.
4
Department of Medicine, University of Alcalá, Alcalá de Henares, Madrid, Spain.

Abstract

BACKGROUND AND PURPOSE:

Different data show an association between human herpesvirus 6 (HHV-6) and multiple sclerosis (MS). Intrathecal anti-HHV-6 immunoglobulin G (IgG) was detected in MS patients, but the antigen recognized by cerebrospinal fluid (CSF) IgG has not been characterized yet. Our objective was to identify the HHV-6 antigens recognized by IgG present in the CSF of patients with MS.

METHODS:

Cerebrospinal fluid IgG of 15 MS patients and eight patients with other neurological diseases was purified on protein G Sepharose columns. Purified IgG from every patient was linked to a CNBr-activated Sepharose 4B column. Fifty micrograms of viral extract was applied to each column. Bound proteins were eluted and analysed by SDS-PAGE and silver staining. The viral protein was characterized by mass spectrometry.

RESULTS:

A protein of 150 kD was eluted from CSF IgG columns of three of eight patients with primary progressive MS and one of seven with relapsing-remitting MS. After digestion and mass spectrometry analysis 10 peptides were found with 100% homology with the major capsid protein of the HHV-6A.

DISCUSSION:

These findings confirm the presence of anti-HHV-6 IgG in CSF of MS patients, particularly in progressive forms, and identify major capsid protein as the major antigen recognized by CSF IgG from MS patients.

KEYWORDS:

antibodies; demyelinating diseases; multiple sclerosis; neuroimmunology

PMID:
24724742
DOI:
10.1111/ene.12435
[Indexed for MEDLINE]

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