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Iran Red Crescent Med J. 2014 Feb;16(2):e14627. doi: 10.5812/ircmj.14627. Epub 2014 Feb 3.

Virulence factors and o-serogroups profiles of uropathogenic Escherichia coli isolated from Iranian pediatric patients.

Author information

1
Department of Pediatric Nephrology, AJA University of Medical Sciences, Tehran, IR Iran.
2
Young Researchers and Elites Club, Islamic Azad University, Shahrekord Branch, Shahrekord, IR Iran.
3
Department of Microbiology, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, IR Iran.
4
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
5
Young Researchers and Elite Club, North Tehran Branch, Islamic Azad University, Tehran, IR Iran.
6
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IR Iran.
7
Young Researchers and Elite Club, Islamic Azad University, Karaj Branch, Karaj, IR Iran.

Abstract

BACKGROUND:

Uropathogenic Escherichia coli O- Serogroups with their virulence factors are the most prevalent causes of UTIs.

OBJECTIVES:

The present investigation was performed to study the virulence factors and O-Serogroups profiles of UPEC isolated from Iranian pediatric patients.

PATIENTS AND METHODS:

This cross sectional investigation was performed on 100 urine samples collected from hospitalized pediatrics of Baqiyatallah Hospital, Tehran, Iran. Midstream urine was collected to decrease potential bacterial, cellular and artifactual contamination. All samples were cultured and those with positive results were subjected to polymerase chain reactions to detect pap, cnf1, afa, sfa and hlyA genes and various O- Serogroups.

RESULTS:

We found that 37.5% of boys and 75% of girls had positive results for Escherichia coli. We also found that O1 (19.33%), O2 (13.33%), O6 (13.33%), O4 (11.66%), and O18 (11.66 %) were the most commonly detected Serogroups. Totally, the serogroup of 5% of all strains were not detected. In addition, all of these O- Serogroups were pap+, cnf1+, hlyA+, and afa+. Totally, pap (70 %), cnf1 (56.66 %), and hlyA (43.33 %) were the most commonly detected virulence genes in the both studied groups of children. The sfa (30 %) and afa (26.66 %) genes had the lowest incidence rates.

CONCLUSIONS:

Special health care should be performed on UTIs management in Iranian pediatric patients. Extended researches should be performed to evaluate relation between other O-Serogroups and virulent genes.

KEYWORDS:

Iran; Pediatrics; Uropathogenic Escherichia Coli

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