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J Biol Chem. 2014 May 30;289(22):15350-62. doi: 10.1074/jbc.M114.549782. Epub 2014 Apr 9.

Inhibition of cellular methyltransferases promotes endothelial cell activation by suppressing glutathione peroxidase 1 protein expression.

Author information

1
From the Cardiovascular and the Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL) and.
2
the Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL) and.
3
From the Cardiovascular and.
4
the Chronic Diseases Research Center, Departamento de Imunologia, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, 1099-085 Lisbon, Portugal.
5
Genetics Divisions, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115.
6
the Molecular Biology of Selenium Section, Mouse Cancer Genetics Program, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, and.
7
the Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL) and Department of Biochemistry and Human Biology, Faculty of Pharmacy, University of Lisbon, 1649-004 Lisbon, Portugal.
8
the Department of General Pediatrics, Center for Pediatrics and Adolescent Medicine, University Hospital, 79106 Freiburg, Germany.
9
From the Cardiovascular and dhandy@rics.bwh.harvard.edu.

Abstract

S-adenosylhomocysteine (SAH) is a negative regulator of most methyltransferases and the precursor for the cardiovascular risk factor homocysteine. We have previously identified a link between the homocysteine-induced suppression of the selenoprotein glutathione peroxidase 1 (GPx-1) and endothelial dysfunction. Here we demonstrate a specific mechanism by which hypomethylation, promoted by the accumulation of the homocysteine precursor SAH, suppresses GPx-1 expression and leads to inflammatory activation of endothelial cells. The expression of GPx-1 and a subset of other selenoproteins is dependent on the methylation of the tRNA(Sec) to the Um34 form. The formation of methylated tRNA(Sec) facilitates translational incorporation of selenocysteine at a UGA codon. Our findings demonstrate that SAH accumulation in endothelial cells suppresses the expression of GPx-1 to promote oxidative stress. Hypomethylation stress, caused by SAH accumulation, inhibits the formation of the methylated isoform of the tRNA(Sec) and reduces GPx-1 expression. In contrast, under these conditions, the expression and activity of thioredoxin reductase 1, another selenoprotein, is increased. Furthermore, SAH-induced oxidative stress creates a proinflammatory activation of endothelial cells characterized by up-regulation of adhesion molecules and an augmented capacity to bind leukocytes. Taken together, these data suggest that SAH accumulation in endothelial cells can induce tRNA(Sec) hypomethylation, which alters the expression of selenoproteins such as GPx-1 to contribute to a proatherogenic endothelial phenotype.

KEYWORDS:

Cell Adhesion; Endothelial Cell; Glutathione Peroxidase; Oxidative Stress; S-Adenosylhomocysteine; Selenoprotein; tRNA Methylation

PMID:
24719327
PMCID:
PMC4140892
DOI:
10.1074/jbc.M114.549782
[Indexed for MEDLINE]
Free PMC Article

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