Format

Send to

Choose Destination
See comment in PubMed Commons below
Adv Virol. 2014;2014:606201. doi: 10.1155/2014/606201. Epub 2014 Feb 26.

Genotyping of HCV RNA reveals that 3a is the most prevalent genotype in mardan, pakistan.

Author information

1
Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan ; Center of Biotechnology & Microbiology, University of Peshawar, Peshawar 25120, Pakistan.
2
Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
3
Department of Biotechnology, KUST, Kohat 26000, Pakistan.
4
Department of Botany, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
5
Center of Biotechnology & Microbiology, University of Peshawar, Peshawar 25120, Pakistan.
6
Department of Agriculture, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
7
Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
8
Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.

Abstract

The clinical outcomes of patients infected with hepatitis C virus (HCV) range from acute resolving hepatitis to chronic liver diseases such as liver cirrhosis or hepatocellular carcinoma. Identification of the infecting virus genotype is indispensable for the exploration of many aspects of HCV infection, including epidemiology, pathogenesis, and response to antiviral therapy. 1419 individuals were screened for anti-HCV in this study, of which 166 (11.7%) were found reactive by ICT (Immunochromatographic test). These 166 anti-HCV positive and 26 normal individuals were further analyzed. RNA was extracted from serum and reverse-transcribed to cDNA and the core region of HCV genome was targeted and amplified by multiplex PCR. HCV RNA was detected in 121 individuals, of which 87 were male and 34 were female. Genotype 3a was the most prevalent among all the genotypes observed followed by 3b. Genotypes 1a, 2a, and 2b were found in 10.89%, 13.22%, and 6.61% patients, respectively. 25.41% of the HCV RNA positive samples were not typed. 6.05% of patients were found having mixed genotypes. These findings will not only help the physicians to prescribe more appropriate treatment for the HCV infection but will also draw the attention of health-related policy makers to devise strategies to curb the disease more effectively.

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Hindawi Publishing Corporation Icon for PubMed Central
    Loading ...
    Support Center