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Electromagn Biol Med. 2015;34(4):285-92. doi: 10.3109/15368378.2014.906448. Epub 2014 Apr 8.

Effects of moderate static magnetic fields on the voltage-gated sodium and calcium channel currents in trigeminal ganglion neurons.

Lu XW1,2, Du L2, Kou L1,2, Song N1,2, Zhang YJ1,2, Wu MK1,2, Shen JF1,2.

Author information

1
a State Key Laboratory of Oral Diseases and.
2
b Department of Prosthodontics , West China Hospital of Stomatology, Sichuan University , Chengdu , China.

Abstract

AIM:

To study the effects of static magnetic fields (SMF) on the electrophysiological properties of voltage-gated sodium and calcium channels on trigeminal ganglion (TRG) neurons.

METHODS:

Acutely dissociated TRG neurons of neonatal SD rats were exposed to 125-mT and 12.5-mT SMF in exposure devices and whole-cell patch-clamp recordings were carried out to observe the changes of voltage-gated sodium channels (VGSC) and calcium channels (VGCC) currents, while laser scanning confocal microscopy was used to detect intracellular free Ca(2+) concentration in TRG neurons, respectively.

RESULTS:

(1) No obvious change of current-voltage (I-V) relationship and the peak current densities of VGSC and VGCC currents were found when TRG neurons were exposed to 125-mT and 12.5-mT SMF. However, the activation threshold, inactivation threshold and velocity of the channel currents above were significantly altered by 125-mT and 12.5-mT SMF. (2) The fluctuation of intracellular free Ca(2+) concentration within TRG neurons were slowed by 125-mT and 12.5-mT SMF. When SMF was removed, the Ca(2+) concentration level showed partial recovery in the TRG neurons previously exposed by 125-mT SMF, while there was a full recovery found in 12.5-mT-SMF-exposed neurons.

CONCLUSIONS:

Moderate-intensity SMF could affect the electrophysiological characteristics of VGCS and VGCC by altering their activation and inactivation threshold and velocity. The fluctuations of intracellular free Ca(2+) caused by SMF exposure were not permanent in TRG neurons.

KEYWORDS:

12.5-mT; 125-mT; calcium channel; sodium channel; static magnetic fields; trigeminal ganglion neuron; whole-cell patch-clamp

PMID:
24712748
DOI:
10.3109/15368378.2014.906448
[Indexed for MEDLINE]
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