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Int J Nanomedicine. 2014 Mar 24;9:1505-17. doi: 10.2147/IJN.S56729. eCollection 2014.

Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat.

Author information

1
Department of Zoology, Faculty of Sciences, Fayoum University, Al Fayoum, Egypt ; Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah Al-Mukarramah, Saudi Arabia.
2
Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.

Abstract

BACKGROUND:

The purpose of this study was to investigate the effect of acute dosing with silver nanoparticles (AgNPs) and identify potential ultrastructural alterations in the liver and kidney and their effect on blood parameters in the albino rat.

METHODS:

Twenty rats were used to assess the acute effects of AgNPs. Rats in the treatment group were injected intraperitoneally with 0.5 mL of distilled water containing AgNPs at a dose of 2,000 mg/kg body weight followed by a second injection after 48 hours. Control rats received two 0.5 mL doses of distilled water only. After 3 days, blood samples were collected, and the rat kidneys and livers were extracted and processed for electron microscopy to investigate for hematologic and histopathologic alterations.

RESULTS:

Renal tubules showed swollen epithelium with cytoplasmic vacuolization, thickening of the basement membrane, and destruction of some mitochondrial cristae. Podocytes showed elongation and swelling of their primary and secondary processes. The basement membrane of the capillary tufts became thicker. The hepatic tissue showed narrowing of the sinusoids, swollen hepatocytes with hypertrophied nucleoli, and accumulation of fat globules in the nucleoplasm and cytoplasm. The hepatic sinusoids showed hypertrophied endothelial and Kupffer. Destructed cristae of some mitochondria, endosomes, and larger lysosomes filled with Ag-NPs were also observed in the Kupffer cells. Significant increases were observed in white blood cell count, lymphocyte count, granulocytes, and hemoglobin. There was a significant increase in serum creatinine, urea, and aspartate and alanine aminotransferases.

CONCLUSION:

To the best of the authors' knowledge, the ultrastructural changes in renal and liver tissue observed in this study have not been described before. Our results suggest that injection of AgNPs could have severe cytotoxic effects on the structure and function of these organs.

KEYWORDS:

blood; kidney; liver; rat; silver nanoparticles; toxicity

PMID:
24711700
PMCID:
PMC3969345
DOI:
10.2147/IJN.S56729
[Indexed for MEDLINE]
Free PMC Article

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