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Cell Metab. 2014 May 6;19(5):810-20. doi: 10.1016/j.cmet.2014.03.025. Epub 2014 Apr 4.

A smooth muscle-like origin for beige adipocytes.

Author information

1
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Cell Biology, Harvard Medical School, Boston, MA 02215, USA.
2
Division of Endocrinology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
3
Department of Anatomy and Cell Biology, Tufts University School of Medicine, Boston, MA 02111, USA.
4
Division of Endocrinology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA; Department of Genetics, Harvard Medical School, Boston, MA 02215, USA.
5
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Cell Biology, Harvard Medical School, Boston, MA 02215, USA. Electronic address: bruce_spiegelman@dfci.harvard.edu.

Abstract

Thermogenic UCP1-positive cells, which include brown and beige adipocytes, transform chemical energy into heat and increase whole-body energy expenditure. Using a ribosomal profiling approach, we present a comprehensive molecular description of brown and beige gene expression from multiple fat depots in vivo. This UCP1-TRAP data set demonstrates striking similarities and important differences between these cell types, including a smooth muscle-like signature expressed by beige, but not classical brown, adipocytes. In vivo fate mapping using either a constitutive or an inducible Myh11-driven Cre demonstrates that at least a subset of beige cells arise from a smooth muscle-like origin. Finally, ectopic expression of PRDM16 converts bona fide vascular smooth muscle cells into Ucp1-positive adipocytes in vitro. These results establish a portrait of brown and beige adipocyte gene expression in vivo and identify a smooth muscle-like origin for beige cells.

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PMID:
24709624
PMCID:
PMC4052772
DOI:
10.1016/j.cmet.2014.03.025
[Indexed for MEDLINE]
Free PMC Article

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