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Early Hum Dev. 2014 Mar;90 Suppl 1:S84-6. doi: 10.1016/S0378-3782(14)70025-8.

Late-onset neonatal group B streptococcal disease associated with breast milk transmission: molecular typing using RAPD-PCR.

Author information

1
Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. Electronic address: m.brandolini@smatteo.pv.it.
2
Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
3
Department of Veterinary Sciences and Public Health, Università degli Studi di Milano, Italy.
4
Neonatology and Neonatal Intensive Care Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Abstract

Group B Streptococcus (GBS) is considered to be the major cause of neonatal sepsis and meningitis of bacterial origin. Late-onset GBS infection is infrequent and occurs between 1 week and 3 months of age. The transmission of GBS through the ingestion of breast milk is reported in the literature, but only a few of these cases have been confirmed by molecular techniques. In this article we report five cases of late-onset GBS disease: transmission through maternal milk was confirmed in four cases, using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) typing assay. In addition, the RAPD-PCR assay showed that each of the isolated clones belonged to a different RAPD genotype, thus revealing that the late-onset GBS infections were not epidemiologically related.

KEYWORDS:

Breast milk transmission; Group B streptococcal disease; Random amplified polymorphic DNA (RAPD)

PMID:
24709469
DOI:
10.1016/S0378-3782(14)70025-8
[Indexed for MEDLINE]

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