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Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:864-7. doi: 10.1016/j.saa.2014.02.171. Epub 2014 Mar 19.

A novel pH optical sensor using methyl orange based on triacetylcellulose membranes as support.

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Department of Chemistry, Faculty of Sciences, Islamic Azad University, Arak Branch, Arak, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, P.O. Box 68149-89468 Khorramabad, Iran. Electronic address:


A novel pH optical sensor based on triacetylcellulose membrane as solid support was developed by using immobilization of methyl orange indicator. The prepared optical sensor was fixed into a flow cell for on-line pH monitoring. Variables affecting sensor performance, such as pH of dye bonding to triacetylcellulose membrane and dye concentration have been fully evaluated and optimized. The calibration curve showed good behavior and precision (RSD<0.4%) in the pH range of 4.0-12.0. No significant variation was observed on sensor response with increasing the ionic strength in the range of 0.0-0.5M of sodium chloride. Determination of pH by using the proposed optical sensor is on-line, quick, inexpensive, selective and sensitive in the pH range of 4.0-12.0.


Methyl orange; Triacetylcellulose membrane; pH optical sensor

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