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Nat Methods. 2014 May;11(5):541-4. doi: 10.1038/nmeth.2922. Epub 2014 Apr 6.

Light-sheet microscopy using an Airy beam.

Author information

1
School of Physics and Astronomy, University of St Andrews, St Andrews, UK.
2
1] School of Physics and Astronomy, University of St Andrews, St Andrews, UK. [2] School of Biology, University of St Andrews, St Andrews, UK.
3
The Scottish Oceans Institute, Gatty Marine Laboratory, School of Biology, University of St Andrews, St Andrews, UK.
4
School of Medicine, University of St Andrews, St Andrews, UK.
5
School of Biology, University of St Andrews, St Andrews, UK.

Abstract

Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

PMID:
24705473
DOI:
10.1038/nmeth.2922
[Indexed for MEDLINE]

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