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Can J Microbiol. 1989 Jan;35(1):189-94.

Presence of an intron in elongator methionine-tRNA of Halobacterium volcanii.

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Department of Medical Biochemistry, Southern Illinois University, Carbondale 62901.


The gene for the elongator (internal) methionine transfer RNA (tRNA(mMet)) in the archaebacterium Halobacterium volcanii contains a 75 base pair intron (intervening sequence) and lacks the 3'-terminal CCA sequence of the mature tRNA. There is a single copy of this gene in the genome and it is expressed. Northern hybridization experiments indicate that the precursor is processed to produce mature tRNA and a free intron species. A secondary structure of the transcript can be formed in which the anticodon stem of the tRNA is extended. Two symmetrically placed three-base bulge loops separated by a 4 base pair stem are present in this extension. The cleavage sites for the removal of the intron are placed between the middle and 3' residues of these loops.

[Indexed for MEDLINE]

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