Format

Send to

Choose Destination
Insect Biochem Mol Biol. 2014 Jun;49:35-42. doi: 10.1016/j.ibmb.2014.03.010. Epub 2014 Mar 31.

Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9.

Author information

1
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
2
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China. Electronic address: xiaqy@swu.edu.cn.

Abstract

Bombyx mori is an economically important insect and a model organism for studying lepidopteran and arthropod biology. Using a highly efficient CRISPR/Cas9 system, we showed that this system could mediate highly efficient targeted genome editing of a single gene locus, large chromosomal deletion or inversion, and also multiplex genome editing of 6 genes simultaneously in BmNs cell line derived from B. mori. The simplicity and high efficiency of our system provide unprecedented possibilities for researchers to implement precise and sophisticated manipulation of a chosen B. mori gene in BmNs cells easily in a limited time course, and perhaps new opportunities for functional genomics of B. mori and other lepidopteran insects.

KEYWORDS:

Bombyx mori; CRISPR/Cas9; Chromosomal deletion; Chromosomal inversion; Multiplex genome editing; Mutagenesis

PMID:
24698835
DOI:
10.1016/j.ibmb.2014.03.010
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center